celltrace (yellow cell proliferation kit)  (Thermo Fisher)

Journal: Frontiers in Immunology

Article Title: Analysis of lipid uptake, storage, and fatty acid oxidation by group 2 innate lymphoid cells

doi: 10.3389/fimmu.2024.1493848

Figure Lengend Snippet: Cytokine production and proliferation of murine ILC2 upon treatment with distinct cytokine combinations. Bone marrow-derived (A, B, E, F) or lung-derived (C, D) group 2 innate lymphoid cells (ILC2) were stimulated with either IL-7 only, IL-33 only, or a combination of IL-7 and IL-33 (A, C, E) or IL-2 only, IL-33 only, or a combination of IL-2 and IL-33 (B, D, F) . All cytokines were applied at 10 ng/mL. (A–D) After 24 hours of stimulation, supernatants were harvested and analyzed for IL-5 content by ELISA. (E, F) Proliferation of ILC2 was assessed using CellTrace Yellow Cell Proliferation Kit after 3 days of incubation with respective cytokines. The data representing the IL-33 stimulation is the same for (A–F) . Data are representative of three independent experiments with stimulations performed in duplicates. Data are shown as average ± standard deviation (SD). Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test (p < 0.01 = ** and p < 0.0001 = ****); ND, not detectable.

Article Snippet: Bone marrow ILC2 were stained with CellTrace Yellow Cell Proliferation Kit (Invitrogen, Catalog No. C34573) according to manufacturer’s instructions.

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Incubation, Standard Deviation

Journal: PLOS Pathogens

Article Title: Isotretinoin promotes elimination of translation-competent HIV latent reservoirs in CD4T cells

doi: 10.1371/journal.ppat.1012601

Figure Lengend Snippet: A: Workflow to identify 5 FDA-approved compounds with transcriptional profiles similar to lead LRA using CMap analysis. Figure created with BioRender. B: CD69 MFI of matched cultured T CM (n = 9) were measured by flow cytometry after treatment with 30 IU/mL IL-2 or IL-2 plus 100 μ M HODHBt or 10 μ M of indicated compounds for 72 hours. Significant p values over cells treated with IL-2 alone. C: Reactivation of latent HIV in T CM (n = 7) measured by flow cytometry after treatment with 100 μ M HODHBt or 10 μ M Isotretinoin alone, plus 100 ng/mL IL-15, or α CD3/CD28. D: Calculation of synergy for LRA combinations using the Bliss independence model (n = 7). Data is presented as the difference between the observed and expected fractional response. E: Viability of uninfected or latently infected matched conditions (n = 7). F: Memory CD4 T cells were stained with CellTrace yellow and treated with 100 μ M HODHBt or 10 μ M Isotretinoin alone or in combination with 100 ng/mL IL-15, or α CD3/CD28 for 7 days. Proliferation was measured by flow cytometry (n = 5). Wilcoxon matched-pairs signed rank test was used to calculate p values (*p < 0.05; **p < 0.01).

Article Snippet: CellTrace Yellow proliferation kit (#C34567) was purchased from Invitrogen.

Techniques: Cell Culture, Flow Cytometry, Infection, Staining